Identification of Leukocyte Surface P2X7 as a Biomarker Associated with Alzheimer’s Disease

Y Li; X Huang; C Fowler; YY Lim; SM Laws; N Faux; JD Doecke; B Trounson; K Pertile; R Rumble; V Doré; VL Villemagne; CC Rowe; JS Wiley; P Maruff; CL Masters; BJ Gu

Journal article

Identification of Leukocyte Surface P2X7 as a Biomarker Associated with Alzheimer’s Disease

Y Li, X Huang, C Fowler, YY Lim, SM Laws, N Faux, JD Doecke, B Trounson, K Pertile, R Rumble, V Doré, VL Villemagne, CC Rowe, JS Wiley, P Maruff, CL Masters, BJ Gu

International Journal of Molecular Sciences | MDPI | Published : 2022

DOI: 10.3390/ijms23147867

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Abstract

Alzheimer’s disease (AD) has shown altered immune responses in the periphery. We studied P2X7 (a proinflammatory receptor and a scavenger receptor) and two integrins, CD11b and CD11c, on the surface of circulating leukocytes and analysed their associations with Aβ-PET, brain atrophy, neuropsychological assessments, and cerebrospinal fluid (CSF) biomarkers. Total 287 age-matched, sex-balanced participants were recruited in a discovery cohort and two validation cohorts through the AIBL study and studied using tri-colour flow cytometry. Our results demonstrated reduced expressions of P2X7, CD11b, and CD11c on leukocytes, particularly monocytes, in Aβ +ve cases compared with Aβ −ve controls. P2X..

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University of Melbourne Researchers

Noel Faux Author

Victor Villemagne Author

Christopher Rowe Author

Paul Maruff Author

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Grants

Awarded by Bethlehem Griffiths Research Foundation

Funding Acknowledgements

This research was funded by ARC Future Fellowship (to B.G., FT120100581), NHMRC Project Grants (1048082, 1061419, 1120095, and 110178 to B.G.), the Ministry of Science and Technology of China (program grant No. SQ2018YFC200022 to B.G.), the Bethlehem Griffiths Research Foundation Grant (BGRF1901 to X.H.), and the Victorian Government's Operational Infrastructure Support Grant to the Florey Institute.